Abul Bashar Ripon Khalipha1*, Sukalyan Kumar Kundu, PhD2, Dr. Md. Nur Alam2, Md. Emdad Hossain, PhD3
1Department of
Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and
Technology University, Gopalganj (Dhaka)-8100, Bangladesh.
2Department of
Pharmacy, Jahangirnagar University, Savar, Dhaka
3Wazed Miah Science Research Center,
Jahangirnagar University, Savar, Dhaka
*Corresponding Author:
Abul Bashar Ripon Khalipha
Email- khalipha1982@gmail.com
ABSTRACT
The
antioxidant capabilities of phenols and flavonoids in medicinal plants help
scavenge reactive oxygen species (ROS). The present study evaluated the
antioxidant and cytotoxic potential of methanolic extracts of Litsea
serbifera also known as Litsea glutinosa
leaves using DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging assay, cupric
reducing antioxidant capacity, nitric oxide scavenging, ferric reducing
antioxidant power, total antioxidant capacity, total phenol and flavonoid
contents, and brine shrimp lethality bioassay. Initial phytochemical analysis
found alkaloid in the extracts. A dose-dependent antioxidant activity was
observed in the fraction compared to ascorbic acid. The crude methanolic
extract had an IC50 value of 385.12 μg/mL in DPPH radical
scavenging experiment, while the reference ascorbic acid had an IC50 value of
9.88 μg/mL. In the nitric oxide scavenging experiment,
the crude methanolic extract had an IC50 value of 60.21 μg/mL, while the reference ascorbic acid had
51.07 μg/mL. A decreased level of antioxidant activity
(36.27±1.39 mg/g equivalent to ascorbic acid) was
detected at 200 μg/mL extract concentration. Extract has high
cupric reduction power and capabilities. The extract included several
flavonoids and little phenols. The MLogP standard and its equivalents are less
than 4.15, indicating that these chemicals are easily absorbed. A therapeutic
candidate molecule's hydrogen bond donor and acceptor should be within five and
ten, respectively. H-bond donor and acceptor of newly developed ligands did not
exceed drug-likeness limit.
 |
Fig. : DPPH scavenging activity of methanolic extract of the Leaves of Litsea serbifera along with the standard
Ascorbic acid (Mean ± SD, n=3) |
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